Leong's Manual of Diagnostic Antibodies for Immunohistology

"The rapid acceptance and entrenchment of immunohistochemistry as an important and, in some cases, indispensable adjunct to morphological examination and diagnosis has imposed the necessity for anatomical pathology laboratories to be proficient in immunostaining procedures. However, for immunohistochemical stains to be meaningful, technical competence must be accompanied by a familiarity with the characteristics and specificities of the reagents employed. In particular, the medical technologist and pathologist must have knowledge of the sensitivity and specificity of the primary antibody employed, the nature of the epitope demonstrated by each antibody and its sensitivity to common fixatives. This book provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, our own experience with these reagents is provided together with pertinent references.While as many available sources of antibodies are provided, it is acknowledged that the listing cannot be exhaustive and only major sources are covered. A brief coverage of the diagnostic approach to the general categories of the poorly differentiated round cell and spindle cell tumors in various anatomical sites using panels of selected antibodies is provided in the form of tables. Staining protocols and antigen/epitope retrieval procedures including those employing enzymes, microwaves and heat are also given in detail"--